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KMID : 0377619670120030287
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Korean Jungang Medical Journal
1967 Volume.12 No. 3 p.287 ~ p.291
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Effect of non-infectious phage lysate on the phage heanagglutination
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Abstract
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The author has investigated the effect of non-infectious phage lysate on phage hemagglutination. For this experiment, the klebsiella phage, it´s host and goat red cells were used and obtained the following results.
By centrifugating of the phage suspension containing about 2x1011/ml of phage for 8 hours at about 17, 000 r.p.m., the phage was almost sedimented and about 7x103/ml of phage was still remained in the supernatent fluid.
The phage was not adsorbed to the sensitized red cells pretreated in non-infectious phage lysate.
When high-titering phage is added to the sensitized red cells pretreated in non-infectious phage lysate, the phage hemagglutination has slightly occurred in higher concentration of antiserum.
The activity of non-infectious phage lysate was neutralized by anti-phage serum and, by diluting, the activity of non-infectious phage lysate became rapidly lowered compared with that of phage under the same grade of dilution.
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